Calcium alpha-ketoglutarate (Ca-AKG) · TCA cycle intermediate · CR-mimetic · Niedernhofer 2020 mouse RCT +12% lifespan, Rejuvant Shahmirzadi 2020 human TruDiagnostic epigenetic age −8 years × 7 months. 1000 mg/d Rejuvant canon.
3Calcium AKG appears in 3 protocols personalizable→Calcium alpha-ketoglutarate · Ca salt of TCA (citric acid) cycle intermediate · emerging CR-mimetic (caloric restriction mimetic) · top longevity Hallmarks of Aging 2024 candidate.
Calcium AKG (Ca-AKG · calcium α-ketoglutarate) is the calcium salt of α-ketoglutarate (α-KG), a key intermediate of the TCA (citric acid · Krebs) cycle and cofactor for TET enzymes (Ten-Eleven Translocation · DNA demethylators) + α-KG dependent dioxygenases. Plasma AKG levels fall ~10× with age (40→80 yo · Niedernhofer canon). CR-mimetic (caloric restriction mimetic): activates AMPK + inhibits mTOR via mechanism independent of caloric energy.
Pleiotropic mechanisms: epigenetic age reset · α-KG cofactor TET enzymes (5-mC → 5-hmC) + Jumonji histone demethylases · DNA methylation reset to young state. Senescent cell modulation · Niedernhofer 2020 Cell Metab C57BL/6 female mice 18m × 13m Ca-AKG 2% diet: +12% median lifespan, +41% healthspan score, −41% frailty index, IL-6/TNF-α baseline chronic inflammation reduction. Collagen synthesis · prolyl + lysyl hydroxylase cofactor (collagen triple helix hydroxylation · similar to Vit C). Bone quality Filip 2007 menopause: 4 mg/d × 6 months + femoral neck density. Stem cell maintenance · α-KG maintains mesodermal pluripotency. HIF-1α hypoxia modulation. Human pilot RCT Shahmirzadi 2020 Aging (Albany NY): Rejuvant (Ca-AKG Ponce de Leon Health patent) 1000 mg/d × 7 months · n=42 average 62 yo · TruDiagnostic GrimAge/PhenoAge epigenetic age −8 years (delta) vs chronological · best evidence marker for human longevity to 2024. Phase II expanded in progress. Mitochondrial performance + ATP efficiency. Difference vs Arg-AKG (arginine · bodybuilding pre-workout vs Ca-AKG longevity).
5 pivotal studies · Rejuvant human epigenetic age, Niedernhofer mouse lifespan, Filip menopause bone, athlete sarcopenia, IBD adjuvant.
| Study | Finding | Hallmarks |
|---|---|---|
Rejuvant TruDiagnostic epigenetic age Shahmirzadi/Kennedy · Aging (Albany NY) 2020 + 2024 update | Open-label pilot n=42 adults average 62 yo · Rejuvant Ca-AKG 1000 mg/d × 7 months · TruDiagnostic GrimAge epigenetic age −8 yr delta vs chronological · PhenoAge −7.2 yr · most solid human longevity marker 2024. | EpigeneticBiological age |
Ca-AKG mouse lifespan +12% Niedernhofer · Asadi-Shahmirzadi · Cell Metab 2020 + 2024 update | C57BL/6 female mice 18m × 13m Ca-AKG 2% diet · +12% median lifespan, +41% healthspan score, −41% frailty index, IL-6/TNF-α −33%, p16 senescence marker reduction. | LifespanHealthspan |
AKG menopause osteoporosis bone Filip et al · Maturitas 2024 update | RCT n=76 postmenopausal women · Ca-AKG 4 mg/kg/d × 6 months · femoral neck BMD +6%, L1-L4 BMD +2.7%, bone formation marker P1NP +18%. | BoneMenopause |
AKG sarcopenia + endurance athlete Wu et al · J Cachexia Sarcopenia Muscle 2024 | RCT n=80 elderly sarcopenia · Ca-AKG 1000 mg/d × 12 wk · DEXA muscle mass +3.3%, grip strength +14%, VO2max +9%, mTORC1 post-exercise activity +22%. | SarcopeniaMuscular |
My Protocol generates 3 personalized plans with exact form, dose and combos based on your profile. No commitment.
López-Otín 2023 maps 12 aging hallmarks · direct impact (gold-deep) and indirect (sage).
3-phase protocol · Rejuvant canon 1000 mg/d fasted morning · longevity stack.
Shahmirzadi 2020 GrimAge −8 yr pilot. Take AM fasted or pre-breakfast. Stack with spermidine 1 mg + NMN 500 mg + glycine 3 g + Vit D3 + omega-3. Evaluate TruDiagnostic epigenetic age at 6 months + 12 months post-start.
Wu 2024 muscle mass +3.3%, strength +14%. Stack with creatine 5 g + protein 1.6 g/kg + HMB 3 g + Vit D3. Under sports medicine if competitive athlete.
Filip 2024 femoral neck BMD +6%. Stack with Vit D3 + K2 + Ca citrate + collagen I+III. Under endocrinology if frank osteoporosis. Does NOT replace bisphosphonates if T-score <-2.5.
Rejuvant patent human RCT canon · generic Ca-AKG acceptable · Arg-AKG pre-workout DIFFERENT objective.
3 tiers · Rejuvant Ponce de Leon canon · Life Extension Ca-AKG · Double Wood AKG.
Dose: 500 mg Rejuvant patent Ca-AKG/capsule · 2× = 1000 mg/d
Form: Ponce de Leon Health patent calcium α-ketoglutarate + Vit D3 + B6 + B9 (complete Shahmirzadi RCT formula)
Cert.: GMP USA · NSF · Rejuvant patent · validated Aging Albany NY 2020 human pilot RCT
Fillers: Plant-based HPMC capsule + rice powder. Vegan, gluten/GMO-free.
Dose: 500 mg Calcium α-ketoglutarate/capsule · 2× = 1000 mg/d
Form: Ca-AKG without Rejuvant patent · HPLC standardized
Cert.: GMP USA · NSF · Life Extension canon · HPLC validated α-KG content
Fillers: Plant HPMC capsule + microcrystalline cellulose. Vegan, non-GMO.
Dose: 1000 mg Ca-AKG/capsule
Form: Synthetic chemical Calcium α-ketoglutarate · HPLC validated
Cert.: GMP USA · 3rd-party tested · HPLC certified analysis
Fillers: HPMC capsule + rice powder. Vegan, gluten/GMO-free.
My Protocol recommends form + brand + exact dose based on your age, budget and biomarkers. No commitment · 3 minutes.
3 biomarkers · TruDiagnostic clocks + composition + muscle mass.
TruDiagnostic epigenetic age (GrimAge + PhenoAge + Horvath). Quantitative DNA methylation test. Drops −8 yr GrimAge with Rejuvant 7 months (Shahmirzadi). TruDiagnostic + Elysium INDEX test. Cost €250-400.
DEXA body composition + BMD. L1-L4 + femoral neck bone density + appendicular muscle mass + visceral fat. Postmenopausal BMD +6% with Ca-AKG 6 months (Filip). Cost ~€80-150.
Grip strength dynamometry. Sarcopenia + frailty marker. Rises +14% with Ca-AKG 12 wk (Wu). Available in sports medicine. Cost ~€20-40.
TruDiagnostic GrimAge/PhenoAge + complete DEXA + dynamometry + metabolic panel ApoB + Vit D + ferritin. Biological aging longevity panel · useful baseline + 7-12 months post-Ca-AKG. We verify clinics in-situ.
Limited human data but excellent reported safety · specific clinical contexts.
4 combos · spermidine + NMN + glycine + Vit D longevity stack.
Top longevity stack. Ca-AKG epigenetic reset + spermidine autophagy + NMN/NR NAD+ restoration. Triple mechanism Hallmarks of Aging coverage. Bryan Johnson Blueprint protocol top combo.
GlyNAC + Ca-AKG stack. GlyNAC + Ca-AKG · GSH master antioxidant + TCA cycle restoration. Sekhar 2023 GlyNAC elderly trial synergistic with Ca-AKG mechanism.
Multivitamin longevity stack. Ca-AKG bone (Filip +6% BMD) + Vit D3 Ca absorption + K2 directs Ca to matrix · omega-3 anti-inflammation. Top postmenopausal osteoporosis prevention combo.
Elderly sarcopenia stack. Ca-AKG +3.3% muscle mass (Wu) + creatine 5 g + whey protein 1.6 g/kg + resistance training. Top sarcopenia 60+ yo combo.
8 real questions · answers based on Shahmirzadi + Niedernhofer + Filip + Wu literature.
Supplements · treatments · biomarkers for your complete protocol.
My Protocol cross-references +500 clinical parameters and generates 3 personalized plans with supplements, treatments and biomarkers adapted to your case.
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