Ozone Therapy in Spain: Prices, Protocol & Verified Clinics

Ozone therapy is a medical treatment that uses a therapeutic mixture of ozone (O₃) and oxygen at controlled concentrations. It is administered via several routes: intravenous (major autohemotherapy), intramuscular, rectal or local. Ozone is a potent oxidant that, at therapeutic doses, activates the endogenous antioxidant system, improves tissue oxygenation and exerts antimicrobial, immunomodulatory and anti-inflammatory effects.

Origin and history

Ozone was first used for medical purposes during the First World War to disinfect wounds. Its systemic use was developed in Germany by Dr Hansler in the 1950s–60s and spread globally from the 1980s onwards.

1. Pre-treatment analysis Mandatory G6PD test. Hepatic and renal function blood panel required prior to treatment.

2. Preparation of the mixture Certified O₃/O₂ mixture prepared using a calibrated generator. Concentration: 20–40 μg/ml.

3. Major autohemotherapy Extraction of 100–200 ml of blood, ozonisation in a sterile glass flask and slow intravenous reinfusion.

4. Monitoring Vital signs monitored throughout reinfusion. Total session duration: 45–60 minutes.

5. Session protocol 10–20 sessions in the initial phase (2–3 per week). Maintenance: 1 session per week.

• Potent systemic anti-inflammatory effect
• Improved tissue oxygenation
• Activation of endogenous antioxidant systems
• Antimicrobial and antiviral effect
• Improved blood circulation
• Support for chronic pain and fibromyalgia
• Immunomodulatory effect in autoimmune conditions

• 1Individuals with chronic inflammation or joint pain
• 2Patients with fibromyalgia or chronic fatigue syndrome
• 3People with chronic infections or Lyme disease
• 4Athletes seeking recovery and performance optimisation
• 5Individuals with peripheral circulatory problems

• G6PD (glucose-6-phosphate dehydrogenase) deficiency
• Pregnancy
• Uncontrolled hyperthyroidism
• Severe thrombocytopaenia

Clinics on LongevityMap declare their exact equipment for objective scoring.

€50–120 per session

10–20 sessions (2–3/week in initial phase); maintenance: 1/week

Prices vary by clinic, equipment and practitioner experience. LongevityMap compares price and quality so you always make the best decision.

The primary mechanism is activation of the Nrf2 transcription factor, which regulates more than 200 antioxidant genes. Studies published in Free Radical Biology and Medicine confirm the induction of HO-1, SOD and GPx following ozone therapy. Major autohemotherapy (AHT) has demonstrated reductions in IL-6 and TNF-α in controlled clinical trials for rheumatoid arthritis and fibromyalgia.