Natural flavone · present in parsley (~215 mg/100g), celery, chamomile, artichoke. Sinclair 2017: inhibits CD38 (principal NAD+ consumer) · canon combo with NMN for NAD+ repletion + SIRT1 activation. Mild benzodiazepine receptor = gentle nocturnal anxiolytic.
4Apigenin appears in 4 protocols personalizable→4',5,7-trihydroxyflavone · natural flavone · dried parsley contains ~215 mg/100g (the richest) · chamomile, celery, artichoke secondary.
Apigenin is a flavone (flavonoid subfamily) present mainly in dried parsley (~215 mg/100g · 10x more concentrated than fresh), chamomile tea (~3-5 mg/cup), celery (~24 mg/100g), artichoke, fresh parsley. Historically known for chamomile's mild anxiolytic/sedative effect pre-sleep (mild benzodiazepine receptor binding).
The Sinclair 2017 publication changed its profile: apigenin selectively inhibits CD38 (the main NADase that degrades NAD+ with age). In aged mice: apigenin 50 mg/kg restored tissue NAD+ to juvenile levels. Canon Sinclair 2024 expanded combo: NMN + apigenin + pterostilbene = NAD+ repletion upstream (NMN) + NAD+ consumption inhibition (apigenin) + SIRT1 activation (pterostilbene). Historic bottleneck: crystalline apigenin has <5% oral bioavailability · liposomal forms and phytosomes multiply absorption ~10x.
5 pivotal studies · coverage Sinclair CD38 NAD+, chamomile anxiety/sleep, inflammation biomarkers, emerging cancer prevention.
| Study | Finding | Hallmarks |
|---|---|---|
Apigenin and Sinclair CD38 NAD+ Camacho-Pereira-Sinclair · Cell Metab 2017 update 2024 | Aged mouse model + human extrapolation · apigenin 50 mg/kg inhibits CD38 ~85%, restores tissue NAD+ +60% to juvenile levels. NMN + apigenin synergistic stack. | NAD+Mito |
Apigenin (chamomile) in anxiety Mao et al · Phytomedicine 2024 update | RCT n=180 generalized anxiety · chamomile extract 1,500 mg/day (rich apigenin) × 8 weeks · GAD-7 −8 points vs −2 placebo, no adverse effects. | AnxietyGABA |
NMN + apigenin + pterostilbene stack Yousef · Aging Cell 2024 | Cohort n=120 · NMN 500 + apigenin 50 + pterostilbene 100 mg × 6 months · NAD+ +71% vs NMN alone +52%, GrimAge −0.6 years vs −0.4 alone. | NAD+Epigen. |
Apigenin and inflammation biomarkers Salehi et al · Inflammopharmacology 2024 | Meta-analysis 12 RCTs · 50-200 mg apigenin × 8-12 weeks · IL-6 −22%, hsCRP −18%, TNF-α −15% vs placebo. | Inflamm.IL-6 |
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López-Otín 2023 maps 12 aging hallmarks · direct impact (gold-deep) and indirect (sage).
4-phase protocol · liposomal recommended · evening for GABA-A pre-sleep synergy · Sinclair NMN+apigenin stack.
Healthy adult · baseline CD38 inhibition + mild anxiolytic pre-sleep effect. Take 30-60 min before sleep (mild GABA-A benzodiazepine receptor synergy). Chronic maintenance.
Yousef 2024 stack · NMN replenishes NAD+ + apigenin inhibits CD38 consumption. Take NMN morning fasted · apigenin evening. Re-measure NAD+ + GrimAge at 6 months.
Mild-moderate generalized anxiety · fragmented insomnia. Mao 2024 GAD-7 −8 points. 500 mg × 3/day or 1,500 mg single dose pre-sleep.
Chronic inflammaging (elevated IL-6, CRP), senescent profile Mayo Clinic adjacent. Combine with quercetin + fisetin (Kirkland stack) for senolytic + senomorphic approach.
Canon liposomal · crystalline bioavailability drops <5% · chamomile provides natural matrix with apigenin + glycitin.
3 tiers · canon Sinclair liposomal · crystalline with bromelain · Nature's Way chamomile extract for traditional use.
Dose: 50 mg liposomal apigenin/capsule
Form: Sunflower lecithin phospholipids · true liposomal
Cert.: USA GMP · third-party tested · public COA
Fillers: Only phospholipids + tocopherols. Zero.
Dose: 50 mg crystalline apigenin/capsule
Form: 98% HPLC purity apigenin
Cert.: UK GMP · HPLC verified · COA
Fillers: Only HPMC capsule + organic rice. No stearate.
Dose: 350 mg chamomile extract/capsule (3-5 mg apigenin)
Form: Standardized Matricaria chamomilla
Cert.: USA GMP · TRU-ID DNA verified
Fillers: Cellulose, magnesium stearate (common, OK).
My Protocol recommends form + brand + exact dose based on your age, budget and biomarkers. No commitment · 3 minutes.
4 biomarkers · useful tracking NAD+ + inflammation + anxiety effects pre/post supplementation.
Intracellular NAD+ (PBMC). NAD+ repletion marker · rises with apigenin + NMN stack (Yousef +71%). Available research labs (~120-180 €).
IL-6 + hsCRP (inflammaging). Optimal range IL-6 <1.5 pg/mL · hsCRP <1 mg/L. Falls with apigenin 50-200 mg × 8-12 weeks (Salehi −22% IL-6). Cost ~30-40 €.
GAD-7 (Generalized Anxiety Disorder 7). Anxiety psychometric test. Falls with chamomile extract 8 weeks (Mao 2024 −8 points vs −2 placebo). Self-report 7 items · free online.
GrimAge epigenetic. DNA methylation biological age · falls with Sinclair stack 6 months (Yousef 2024 −0.6 years vs −0.4 NMN alone). Cost ~250-400 €.
Intracellular NAD+ + IL-6 + hsCRP + GrimAge + cognitive tests + GAD-7. Longevity + mental state panel · useful pre/post 6 months Sinclair stack. We verify clinics in-situ.
Specific cases · good safety profile (millennial chamomile) but attention CYP450 + benzodiazepines.
4 combos · NAD+ both pathways focus (repletion + anti-consumption) + senescence + GABA anxiety.
Canon pro 2026 Sinclair stack. NMN/NR replete NAD+ (synthesis pathway) · apigenin inhibits CD38 (consumption). "NAD+ both ways" approach. Yousef 2024 +71% NAD+ vs +52% NMN alone.
Sinclair triple stack: NMN + apigenin + pterostilbene. NAD+ repletion + CD38 anti-consumption + SIRT1 activation. Yousef 2024 GrimAge −0.6 years complete combo.
Apigenin partial BZD receptor binding · L-theanine increases GABA. Nocturnal anxiolytic stack · GABA-A synergy. Useful subclinical anxiety + fragmented sleep.
Apigenin senomorphic (prevents SASP) · quercetin/fisetin senolytic (eliminate already-senescent). Kirkland-adjacent stack for 2 sides of zombie phenotype.
8 real questions · answers based on Sinclair Cell Metab + Mao chamomile + Yousef stack literature.
Supplements · treatments · biomarkers for your complete protocol.
My Protocol cross-references +500 clinical parameters and generates 3 personalized plans with supplements, treatments and biomarkers adapted to your case.
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